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However, our internal experience suggests that for the most sensitive research applications, potency can begin a measurable decline even before that point

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The following primary antibodies were used: Phospho-AKT (p-AKT)-S473 (1:200, #4060, Cell Signaling, Bioconcept, Allschwil, Switzerland), NRF2 (bs-1074, 1:200, Bioss Inc, Lucerna-Chem AG, Luzern, Switzerland), Phospho-AMPK1/2 (p-AMPK)-T183/172 (1:200, STJ90735, St Johns Laboratory, Lucerna-Chem AG)