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To further confirm metabolic competence in selected liver cell models (PHH, HepG2 cells), CYP mRNA expression was investigated by quantitative polymerase chain reaction (qRT-PCR)

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MRP2, Multi-drug Resistance-associated Protein 2

Studies have shown significantly reduced GPX3 mRNA levels in chondrocytes from KBD patients, where elevated methylation of CpGs in the GPX3 gene suppresses its transcription, reducing antioxidant function and promoting chondrocyte apoptosis, thus accelerating KBD progression [205,206]

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