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A similar UA cytotoxicity was reported by Figure 3, the cytotoxicity of the LNCs was concentration-dependent

Membranes in balance: mechanisms of sphingolipid homeostasis
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Its a critical component of cell membranes
To chase the HADA staining, cells were harvested at 4,000 g for 5 min, washed once by resuspension in 8 ml reduced TY and finally resuspended in 2 the original volume of reduced TY media before continuing growth for up to 30 min