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The content was collected, filtered through a 70 m cell strainer and red blood cells were lysed using Red Blood Cell Lysis Buffer (Sigma-Aldrich) according to the manufacturers protocol

Severe selenium deficits in pregnant women irrespective of autoimmune thyroid disease in an area with marginal selenium intake
Moreover, insulin-like growth factor binding protein related proteins (IGFBPrPs) have been recently characterised, which aid the metabolic effects of the hormone but their role remains unclear [96, 97]

Cross N, Mahasreshti P (1997) Prostasome fraction of human seminal plasma prevents sperm from becoming acrosomally responsive to the agonist progesterone
