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If you have symptoms of vitamin B12 deficiency, you should see a medical provider for a proper diagnosis, which usually involves blood testing

The injection would be given slowly into a vein, or added to an IV drip

There is, per ClinicalTrials.gov, no completed human efficacy trial for 5-Amino-1MQ

The generation and functional characterization of induced pluripotent stem cells from human intervertebral disc nucleus pulposus cells