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N Engl J Med 1981

2.6 Real-time PCR analysis Total RNA was prepared from liver tissues and LO2 cells using the simple RNA Extract kit (Tiangen Biotech, China) and reverse transcription of RNA to cDNA using Fast King cDNA First Strand Synthesis Kit (Tiangen Biotech, China)

However, with changes in the social environment, many mothers cannot breastfeed their infants due to insufficient lactation, work commitments, or other reasons, and instead use commercially available formula for artificial feeding