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Inhaled nitric oxide reaches the alveolus and dilates the pulmonary vasculature

doi: 10.1128/IAI.72.7.3941-3950.2004 23 KappaunK.PiovesanA

doi: 10.1111/bjd.18245 4 NowowiejskaJBaranAFlisiakI

We used the Click-iT Nascent RNA Capture assay in HepG2 cells incubated in the presence or absence of NaOX, with or without the alkyne-modified nucleoside 5-ethynyl uridine (EU), which has a tenfold higher affinity for mRNA incorporation over endogenous uridine and, thus, possesses high sensitivity via bio-orthogonal approaches