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The overlaps between differentially expressed genes in each transcriptome dataset were evaluated as Venn diagrams ( and Upset plot ( RT-qPCR Total RNA was extracted from 3040 mg leaf tissues with Sepasol-RNA I Super G and the TURBO DNase free kit (Thermo Fisher Scientific) according to the manufacturers protocols, followed by reverse transcription with the PrimeScript RT reagent kit (Takara Bio, Shiga, Japan) using oligo dT primers
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