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After washing the plates five times with TBS + 0.05% Tween-20, plates were developed by adding develop solution (1% 3,3,5,5-tetramethylbenzidine (TMB, Sigma-Aldrich), 0.01 % H 2 O 2 , 100 mM sodium acetate, 100 mM citric acid) and the reaction was stopped after 3 min by adding 0.8 M H 2 SO 4

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Surprisingly, no established criteria were available to comprehensively characterize the expanded chondrocytes before they are implanted

Participants exhibited reduced neural response to high-calorie food images, suggesting a shift in food-related attention and motivationmechanisms that may contribute to sustained appetite suppression and reduced caloric intake in research contexts