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Ice crystal formation during freezing physically damages the peptide structure in solution

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Imbalance in mitochondrial fission and fusion is a key trigger for Parkinsons disease, and defects in mitochondrial dynamics limit mitochondrial movement, leading to a reduction in ATP, excessive oxidative stress, and mtDNA deficiency, which ultimately leads to cell death (Geng et al., 2019)

The calculation is: PTE = 0.5 (IND period) + 1 (NDA review period), subject to a cap of five years and a maximum remaining patent term of 14 years post-approval