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doi: 10.1038/nri2161CrossRefGoogle ScholarPubMed Liang, Y, Bruce, J, and Boyd, D (1987) Laboratory cultivation of schistosome vector snails and maintenance of schistosome life cycles

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Sperm motility, viability (live/death ratio) and morphology (normal and abnormal [head defect]) were analyzed with a microscope (Leica DM750) and reported as the mean percent of motile sperm according to the method developed by the World Health Organization.25 Sex hormone and pituitary gonadotropins determination The serum levels of Testosterone (TT), Follicule stimulating hormone (FSH), Leutenizing hormone (LH) and estrogen were measured using commercially available enzyme-linked immunoassay kits (Diagnostic automation Inc, CA) obtained from Randox Laboratories Ltd., Admore Diamond Road, Crumlin, Co., Antrim, United Kingdom, according to the manufacturer's instructions