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However, ipamorelin does not increase ghrelin levels

Here, we discuss allosteric networks and allosteric signaling bias

Separation of soluble and insoluble components Single mouse hippocampal tissue was isolated, and 300 l mixed protein lysis solution with RIPA (Applygen, C1053), a protease inhibitor (Merck, 539134), and a phosphatase inhibitor (Merck, 524628) (=100:1:2) were added

Tissue sections were imaged under a microscope (DMi8, Leica Microsystems, Wetzlar, Germany, with a Hamamatsu Orca Flash 4 v3 sCMOS camera) where fluorescence was excited with a lumencor spectra X using 550 nm (secondary antibody Alexa Fluor 594), 470 nm (secondary antibody Alexa Fluor 488) or 385 nm (DAPI), and exposure time was held constant for each color channel regarding magnification and staining

Brain-derived neurotrophic factor (BDNF) is required for the enhancement of hippocampal neurogenesis following environmental enrichment