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The FOXO3a protein level is downregulated in cetuximab-resistant CRC cell lines Next, we investigated how KRAS/BRAF mutations affect FOXO3a activity and how FOXO3a is regulated in CRC cells with acquired cetuximab resistance
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Subsequently, the cells were washed with PBS and blocked for 1 h with 1% bovine serum albumin (BSA, Sigma A9418) and 22.52 mg/mL glycine in PBST (PBS + 0.05% Tween-20, LPS Solution CBP007T)
Im et al, 2011
