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First, TAT-CcSOD1 (200 g/mL) was dissolved in various buffers including 50 mM acetate buffer (pH 3.06.0), sodium phosphate buffer (pH 6.08.0), TrisHCl buffer (pH 8.09.0) and glycineNaOH buffer (pH 9.011.0) and incubated at 37 C for 2 h