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Am J Ind Med 1994, 25 (1), 41-2

The effect of metals and inhibitory compounds were tested using a 150 l reaction mixture containing 1 g of protein in 50 mM Tris-Base, 150 mM NaCl, pH 7.5 and the different metals or inhibitory compounds (metals and inhibitory compounds at 10 mM, except FeCl 3 at 1 mM) and incubated for 30 min at room temperature, followed by the addition of p NPP to a final concentration of 500 M and further incubated for 30 min at 37 C

too short, and cells stop replicating

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Nutritional status and overall health influence results