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Immunofluorescence Liver frozen sections and cells were performed as described previously (Seidel et al ., 2021)

Abdominal distension was the most common symptom at a prevalence of 92%, while postprandial fullness, diarrhea, abdominal pain, and constipation were reported in 55-73% of cases

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1H) due to extended periods where oligodendrocyte loss exceeded 1.5% per day (Supplementary Fig

Real-Time PCR Total RNA was extracted from cells by acid guanidinium thiocyanate-phenol-chloroform extraction method, and complementary (c)DNA was synthesized using 1 g total RNA in a 20 L RT reaction mix containing 0.5 g/L of random primers, 0.1 mM dNTP, 0.1 M DTT and 5 first strand buffer
