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By blocking the activity of NNMT, 5-Amino-1MQ increases the bioavailability of nicotinamide, a precursor to NAD+, thereby elevating intracellular NAD+ levels

Importantly, animals were singly housed to eliminate the exchange and cross-contamination of intestinal microbiomes, a factor that potentially biases studies with group-housed animals by minimizing microbiome variations for co-housed animals within a cohort

Furthermore, a significant positive correlation was observed between ScGPAT2 and glucose ( r = 0.222, p = 0.019) (Additional file 1: Table 1)