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Desalt the supernatant with PD-10 columns equilibrated with 100 mM NaCl containing 20 mM Na + /K + phosphate buffer pH 7.4 (or 20 mM Tris base for rat RBCs)

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Chronic Kidney Disease Patients with AKI may gain normal kidney function but have an increased risk of developing progressive CKD [189,190,191]

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