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-L-glutamyl-L-cysteine can be a substrate for -glutamylcyclotransferase (GCT), however, due to higher affinity and activity of glutathione synthetase (GS), glutathione synthesis is favored in animal cells.[118] In the presence of GS and ATP, -L-glutamyl-L-cysteine is phosphorylated to form enzyme bound -glutamyl-cysteinyl-(P), which reacts with Gly to liberate free glutathione and Pi.[9] This pathway occurs virtually in all cell types (except epithelial cells), with liver being the major producer and exporter of glutathione

Ji, T., Huang, G., Cao, Y., Gao, Y

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3 University Medical Center of El Paso, Patient Blood Management, El Paso, United States

Superoxide anion (O 2 - ), hydroxyl (OH ), alkoxy radicals (RO ), peroxyl radicals (ROO ), nitric oxide radical (NO ), nitrogen dioxide (NO 2 ), and carbonate radical (CO 3 - ) are the few examples of radical ROS whereas non-radical ROS include singlet oxygen ( 1 O 2 ), hydrogen peroxide (H 2 O 2 ), hypochlorous acid (HOCl), and peroxynitrite (ONOO - ) species which can readily trigger free radical reactions in organisms [23, 24]