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Since mobile phase containing 0.1% OPA has a higher pH than the pKa of the NAC carboxylic group and placebo components, there are insufficient protons (H + ) in solution, and NAC dissociates into its conjugate base and become ionized, resulting in reduced retention, on RP-HPLC

10.1016/j.drugalcdep.2016.08.485 Drug Alcohol Depend