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To test this we used quantitative RT-PCR (QPCR) to measure gene expression with primers designed to amplify fat-5, fat-6, and fat-7, along with the control genes tbb-2 (-tubulin) and ubc-2 (ubiquitin-conjugating enzyme, E2)

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Among the altered genes, we identified expression differences in genes encoding key enzymes of lipid metabolism (Fig 5C and D)