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For GST-tagged AcvB protein purification, cells were resuspended in buffer A (20 mM Tris-HCl [pH 8.0] and 150 mM NaCl) supplemented with 5 mM dithiothreitol, disrupted by sonication, and centrifuged at 20,000 g for 40 min to remove the insoluble debris

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PMID: 23658863

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Glutatin reducido: Forma activa del glutatin que participa en mltiples funciones celulares clave, ayudando a neutralizar radicales libres y favorecer la regeneracin de otros antioxidantes