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The media was then replaced with fresh media and treated with 25 M acivicin, 100 mM 2-imidazolidone-4-carboxylate (2I4C), 200 M buthionine sulfoximine (BSO) or 5 M pyroglutamate (PGA) for glutathione and glutamate measurement

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Then, a GSH reacts with the oxidized enzyme and generates an intermolecular selenylsulfide bond

Malaysian Orthopaedic Journal, 15 (2), 18