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It involves extraction, reduction (with immobilized tris(2-carboxyethyl)phosphine, TCEP), deproteinization and concomitant fractionation of GSH using size exclusion chromatography (Fig

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2.1.2 Statistical analysis Plasma potassium parameters were calculated using the WinNonlin 7.0 Software (Pharsight Corporation, Mountain View, CA, USA) via non-compartmental modeling

Moreover, histone modifications also serve a vital role in the ability of TME to adapt to changes in the microenvironment (e.g., nutrient availability and oxygen levels) by regulating expressions of genes involved in cell survival and proliferation in hypoxic conditions of TME [68,79]