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In summary, our results demonstrated that TG2 in cell lysates causes GST aggregate formation through crosslinking activity, which interferes with pulldown experiments and alters fused protein function

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Generation of MRP1 and MRP2 vectors ABCC1 gene was subcloned from a previous pcDNA3.1(-)-MRP1, using the restriction enzyme XbaI and NotI to extract the ABCC1 insert