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Briefly, the peptide samples were trapped and washed on Pepmap100 C 18 trap (5m, 0.3 5 mm) at 20ul/min using 2% acetonitrile in water (with 0.1% formic acid) for 10 min and then separated on a Pepman100 RSLC C18 column (2.0 m, 75-m 150-mm) using a gradient of 2%40% acetonitrile with 0.1% formic acid over 120 min at a flow rate of 300 nL/min and a column temperature of 40C

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Pre-treatment with sarcosine (300500 mg/kg, i.p.) for 7 days before global ischemia (4VO model) in rats resulted in significant neuroprotection, as evidenced by reduced neuronal loss, infarct volume, and cognitive impairment (Pinto et al

These milk oligosaccharides selectively nourish bifidobacteria species, and starve the strains of bacteria that are harmful, leading to an optimized microbiome in the gut and throughout the entire body

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