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LC-MS-based metabolite detection For determination of the relative Cys and GSH pool sizes across compartments, previously established LC-MS conditions were employed 5,24 For chromatographic metabolite separation, a Vanquish UPLC system was coupled to a Q Exactive HF (QE-HF) mass spectrometer equipped with HESI (Thermo Fisher Scientific, Waltham, MA, USA)

Because phi and tau GSTs are the most abundant classes in plants, the results described above indicate variable inhibition by natural compounds per GST class should be expected in animals versus plant systems

Specific gamma-hydroxybutyrate-binding sites but loss of pharmacological effects of gamma-hydroxybutyrate in GABA (B)(1) - deficient mice