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Following a 3-day incubation, cell viability was assessed using a Cell Titer-Glo Luminescent Cell Viability Assay (Promega Corporation)

The smaller GSH-capped QDs increased the activity by 9%, via partially restoring JBUs -helical content

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The incubates were then spun through Bio-Gel P6 spin columns (400 packed volume) for 1 min to separate free and bound 3 H-Hinge and the eluent was collected

On this basis, Rayman proposed that 122 g/L represents the concentration of baseline serum selenium that delineates a change in risk, from lower to higher, of developing cancer and type 2 diabetes in patients treated with selenium supplementation of 200 g per day (50)