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Description
High-throughput automated preparation and simulation of membrane proteins with HTMD
Selected Area Electron Diffraction (SAED) patterns of zone axes were recorded
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After centrifugation at 65,000 g for 30 min, the supernatant was collected and incubated with 10 ml Ni-NTA resin (Cytiva) in the presence of 8 mM imidazole to prevent the nonspecific binding at 4 C overnight
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