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Pharmacological inhibition of this pathway by agents such as erastin depletes GSH reserves, directly inactivating GPX4 through loss of reducing power and culminating in uncontrolled membrane lipid peroxidation ( 3.3.2 Nrf2/ARE axis Transcription factor Nrf2 is a core regulator of cellular oxidative stress responses

10.1074/jbc.M113.513184 40 DeniaudA.Sharaf El DeinO.MaillierE.PoncetD.KroemerG.LemaireC.et al (2008)

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After three washes with free-serum media, cells were collected, counted, and analyzed for their fluorescence intensity using a fluorescence microplate reader (Thermo Fisher Scientific, Waltham, MA, USA) with its excitation and emission wavelengths at 488 nm and 525 nm [29, 30]

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