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Briefly, cells were grown in YPD until logarithmic phase, harvested by centrifugation at 1000 g for 3 min at room temperature, and resuspended in 200 l of One-step-transformation buffer containing 40% polyethylene glycol 3350 (Sigma Aldrich, St

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Vitamin B6 forms red blood cells, helps cells make protein, produces neurotransmitters, such as serotonin and releases stored forms of energy, helps prevent CVS disease and strokes, helps relieve depression, and alleviates insomnia

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