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Materials and Methods Experimental Animals and Groups In our study, we raised 120 specific pathogen-free (SPF) grade Wistar male rats (180 20 g weight and 24 weeks old, which is equivalent to 1820 years old in humans) provided by the Experimental Animal Center of the 940th Hospital of Joint Logistics Support force of Chinese Peoples Liberation Army (licence: SCXY (Military) 20120020)

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The gfp surface object was selected and the total gfp signal was pulled from the statistics tab (Detailed tab, select Average Values from dropdown, Intensity Sum Ch = 4 Img = 1 row and Sum column) and stored in an excel file

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