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The forward and reverse primers were mixed with 100 ng cDNA and quantitative RT-PCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems, Darmstadt, Germany) or THUNDERBIRD SYBR qPCR Mix (TOYOBO, Japan)

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M., & Langendonk, J

Increases metabolism : This helps pets burn more calories, even at rest, improving weight management

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