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Although external stimuli induce ChaC1/ChaC2 gene and GGT expression (15,174,175), the specific regulatory mechanisms governing these inductions remain unclear

Several groups have also combined sod mutations with other long-lived mutants and examined the effects on lifespan

Nature Aging 2021;1, 1117-1126, doi:10.1038/s43587-021-00151-2

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Indirect immunofluorescence analyses Cells grown on collagen/fibronectin coated coverslips were fixed with formalin for 15 minutes, permeabilised with 0.1% Triton X-100, blocked with 2% FBS/HBSS and incubated with primary antibody for 1 h followed by washing and Alexa-conjugated secondary incubation, further washes, nuclear staining with 1 ug/ml DAPI and mounting