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It is composed of a highly viscous nucleus pulposus surrounded by a thick fibrocartilage outer ring, annulus fibrosus, and sandwiched by cartilage endplates below and above (Fig
Each 40 l reaction contained 2 l of the first-round PCR product, 1 l each of 10 M P5 and P7 custom index primers, 0.4 l of Phusion High-Fidelity DNA Polymerase (NEB, no

1.1 ENS serves as the primary regulator of intestinal motility and secretory function ENS functions as the central integrative regulator of gastrointestinal motility and secretory activity, coordinating complex sensorimotor programs through a highly specialized neuromodulatory network
