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l- or d-carnitine (04 g/kg diet) was fed to the low-carnitine tilapia for 6 weeks

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These may arise indirectly from rapid weight loss-induced metabolic shifts, including nutrient and electrolyte depletion (e.g., magnesium, potassium, B-vitamins, iron), mitochondrial energy stress, and release of stored fat-soluble toxins (environmental chemicals, pesticides) into circulation during adipose tissue breakdown

Finally, the ImageJ software was used to count apoptotic cells