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Gao Y, Martin NI, Van Haren MJ (2021) Nicotinamide N -methyl transferase (NNMT): an emerging therapeutic target

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Steps: Gather supplies: AOD9604 5mg vial(s) Sterile water (0.9% benzyl alcohol) Sterile syringes (1mL or 3mL) Insulin syringes for administration (0.3mL or 0.5mL) Alcohol swabs Prepare the vial: Remove the plastic cap from the vial Swab the rubber stopper with alcohol Let it air dry completely Add sterile water: Draw the desired amount of sterile water into the syringe Common volumes: 12 mL per 5mg vial Insert the needle through the rubber stopper Inject the water slowly down the side of the vial, not directly onto the powder Mix the solution: Gently swirl the vial in a circular motion Do not shake vigorously (it can damage the peptide structure) Let the powder dissolve completely (12 minutes) The solution should be clear and colorless Calculate concentration: Example: 5mg AOD9604 + 2mL sterile water = 2.5mg/mL Example: 5mg AOD9604 + 1mL sterile water = 5mg/mL Use the Peptide Calculator for precise figures Reconstitution tips: Use 2mL sterile water for easier dosing calculations Label the vial with the reconstitution date and concentration Refrigerate the reconstituted solution immediately Use within 28 days for best potency Administration Technique Proper subcutaneous injection technique ensures good absorption and minimal discomfort