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The GAPDH gene was served as the internal control ( CT method ( Cloning, expression and identification of rSmGST The first Spirometra GST gene deposited in GeneBank (AEI16476.1) was amplified by PCR with specific primers carrying BamHI and PstI restriction enzyme sites (underlined) (forward, 5-AT GGATCC ATGGGTTC GCTCCCGGTTC-3, and reverse, 5-AT CTGCAG CTAAGCATCACCACGCCAG-3), and the cycling protocol was as follows: 30 cycles of 95C for 50 s, 60C for 50 s and 72C for 50 s

doi: 10.1038/s41467-022-30623-x 113 RizigM.Bandres-CigaS.MakariousM

Biochemistry, pathophysiology, and regulation of linear ubiquitination: intricate regulation by coordinated functions of the associated ligase and deubiquitinase

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Whitmore et al