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Most of these PSD-95 positive spines are stable, but, in the rare cases that they lose PSD-95 and disappear, or are formed de novo and gain PSD-95, their dynamics result in a persistent synapse gain or loss and permanent circuit rewiring associated with learning and memory, respectively

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Importantly, while most vertebrate species can synthesize ascorbic acid, humans cannot, and they are therefore dependent on oral consumption of VitC

In particular, the recent emergence of base and prime editing offers precise, double-strand break-free strategies to engineer these functional modifications, highlighting the ongoing evolution of genomic tools for enhanced neuronal differentiation and the potential application of CRISPR-based therapeutic approaches to improve cell replacement therapy with increased safety in the clinic 102,103