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After each analysis, the column was washed for 10 min with 20% buffer A and 80% buffer B
It is a coherent rationale
Relative gene expression was calculated using the 2^ Ct method, where Ct = Ct of target gene Ct of reference gene, and Ct = Ct of experimental group mean Ct of control group
Effectiveness of food fortification in the United States: the case of pellagra
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