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These changes are long term regulatory effects

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2.10 Identification of reaction products by HPLC Samples from MSA-DH assays, from MDH assays or from pathway reconstitution experiments were separated on a Repromer H column (300 4,6 mm, 9 m, TECHLAB GmbH) using a HPLC-system equipped with a degasser (DG-1580-54, Jasco), a gradient unit (LG-1580-04, Jasco), a pump (PU-1580, Jasco), a sampler (AS-1555, Jasco), a column oven (CO-1560, Jasco) and a multi wavelength detector (MD-1515, Jasco)