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In the ATP-bound conformation, the mutation of Gly to Asp will have limited expected effects, resulting most probably in a rotamer rearrangement in respect of D999 with limited consequences on the backbone position

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Additional TdT reaction buffer can be purchased as Cat

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Targeted Methods for Capturing and Cloning BGCs Despite the continued use of cosmids and BACs in cloning BGCs, newer technologies have been developed to be more user-friendly