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In addition to somatic mutations in Keap1 , hot-spot mutations in the NFE2L2 gene in regions encoding the ETGE and DLG motifs in Nrf2 that bind to Keap1 have also been reported [383, 406410]

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76,88 In contrast, direct free T4 assays separate free and protein-bound T4 using equilibrium dialysis or ultrafiltration, and use liquid chromatography tandem mass spectrometry or radioimmunoassay to measure free T4

PLoS ONE 4 , e4715 (2009)

Sources of HMs responsible for creating HMs toxicity to soils (A)