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A meta-analysis of 87 studies (9,109 participants) found that ASD children had significantly elevated blood levels of oxidative markers such as GSSG, malondialdehyde, and homocysteine, while significantly decreased levels of GSH, total glutathione, the GSH/GSSG ratio, and antioxidant substances such as cysteine, vitamin B9, and vitamin D

According to the NIH, these doses are safe as vitamin B12 is a water-soluble vitamin of which your body will only absorb what it needs and excrete the rest

This can be explained by the heterogeneity of the studied population

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Even if you only used it once, that needle is no longer sterile