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Appropriate formulation and use which is supervised by a knowledgeable healthcare professional will maximize the benefits while minimizing any potential side effects of these therapies

For HPLC measurement, a mixture of acetonitrile and 0.1% formic acid solution (95: 5, v/v) was used as an eluent at a flow rate of 0.3 mL/min

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Many drugs enter the BBB primarily through the transcellular pathway, so we can determine the physiological functional integrity of the in vitro model by immunofluorescence techniques to detect the amount of several membrane inward and outward transport proteins, such as GLUT-1, the transferrin receptor or P-glycoprotein[208]