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RT-PCR reactions were performed in a total volume of 25 l containing 25100 ng of total RNA, 200 m of each dNTP, 1.5 m MgCl 2 , 25 pmol of each primer, 2.5 U of AMV RT and 2.5 U of TFL DNA polymerase (Access RT-PCR System, Promega)

A functional genetic variation of adenosine deaminase affects the duration and intensity of deep sleep in humans

The research employs a randomized complete block design, featuring four treatments (control, biochar, cover crop, and synthetic nitrogen), replicated twice at each site on 4-acre plots

[DOI] [PubMed] [Google Scholar] 48.Birk J., Meyer M., Aller I., Hansen H.G., Odermatt A., Dick T.P., Meyer A.J., Appenzeller-Herzog C

Similar observations were made in MDA-T32 and MDA-T68 PTC cell lines (Shown in Supplemental Fig