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As expected, T cell-independent (TI) type II immunization with TNP-Ficoll to evaluate MZB-dependent antigen binding and early antibody production 86,87,88 confirmed the deficit of MZB in Gclc fl/fl Mb1-Cre + mice (Supplementary Fig

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2.15.2 Neural function defect score (NSS) Twenty-four hours post-infarction, neurological deficits were scored using the Masao Shimizu Sasamata method ( 2.16 Immunohistochemical analysis Mice were humanely sacrificed by deep anesthesia using an overdose of pentobarbital, followed by transcardial perfusion with phosphate-buffered saline (PBS) to clear the blood