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Nickel affinity chromatography The nickel on agarose beads binds to proteins through polyhistidine tags, known as his-tags, that usually range from 6 to 10 histidine residues long (Figure 2)

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Aims To investigate constituent amino acid plasma levels and the GSH redox status in different compartments in IBD with emphasis on intestinal GSH synthesis in Crohns disease

The resulting co-expression genes modules and hub genes were generated based on the weighted gene co-expression network analysis and visualization by Cytoscape