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Elpeleg ON, Hammerman C, Saada A, Shaag A, Golzand E, Hochner-Celnikier D, Berger I, Nadjari M

For RNA-Sequencing of the cDNA, we hybridized 5 pM of each library to a flow cell, with a single lane for each sample, and we used an Illumina cluster station for cluster generation

Under energy stress conditions, AMPK directly phosphorylates the autophagy-initiating factor ULK1 at serine residues 317 and 777, promoting autophagosome formation ( 4.5 The interaction network of AMPK with other metabolic pathways The functional roles of AMPK extend beyond its classical functions to dynamic interactions with other metabolic pathways

Transfection of siRNAs C2C12 cells were treated with 100 ng/mL TNF- for 2 h to establish the muscle atrophy cell model