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Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Standard Curve This product is detected by QC department and meets performance required in the manual

Cell culture The 46BR.1N cell line (Sigma Aldrich), HaCaT cell line (DKFZ, Heidelberg, Germany) 14,15 and primary dermal fibroblasts were cultured in DMEM HG with 10% FBS and 1% PS umder standard conditions (37 C, 5% CO 2 )

doi: 10.1016/j.drudis.2020.11.002

The contribution of each of these interactions to 2 -AR endocytosis was assessed using -Arr mutants deficient in either AP-2 or clathrin binding

Step-by-Step Guide: Best Practices for BPC-157 Use Consult a Healthcare Professional: Before beginning any peptide therapy, discuss your health goals and medical history with a qualified provider